Light sheet for the masses
More than a hundred years ago in Jena a physicist and a chemist combining their efforts created the first light sheet for imaging. Their efforts won them the Nobel prize in 1925 but their idea of uncoupling illumination and detection was lost. But with better laser illumination and electronic devices, this basic principle has become a revolution in biological imaging since its revival in 1993. It is now available for histology, pathology, developmental biology, plant biology, cell biology as well as cytometry. So far more than 80 acronyms described at least one type of light sheet microscope, but is it that amazing? The talk will walk you through history and the jungle of variations around the same theme and how this technology will affect biological imaging as well as cytometry in the coming decade.
Dr. Emmanuel G. Reynaud, MSc, BSc, PhD, is a Lecturer in Integrative Biology at the UCD School of Biomolecular & Biomedical Science. Prior to joining UCD, he was a Researcher and Postdoctoral Fellow at the European Molecular Biology Laboratory, where his research focused on development of new imaging methods (e.g. Light Sheet Microscopy) and optical micromanipulations in Cell Biology (e.g. laser nanosurgery), in the laboratory of Prof. Ernst H.K Stelzer and Dr Rainer Pepperkok. He is also the co-founder of the Light Sheet Microscopy community alongside Dr Pavel Tomancak. He also built and coordinated for 2.5 years a unique imaging platform during the circumnavigation of the Earth as part of the Tara Oceans (2009-2012). He has been awarded a Knight of Palmes academiques for his educational works.