Raman microspectroscopy – a new tool for cultivation-free pathogen identification

Dr. Petra Rösch
Institute for Physical Chemistry

For severe infectious diseases standard diagnosis methods like PCR or microbiology taking 8 h or even several days are too long for a targeted antibiosis. Therefore, point of care diagnostics like Raman microspectroscopy, which do not require any cultivation at all, are indispensable.

Raman microscopy achieves a lateral resolution of approximately 1 µm thus allowing the analysis of single bacterial cells. With this phenotypical technique the biochemical content of a cell is analyzed. In this tutorial we will demonstrate the capability of Raman spectroscopy to identify pathogens without cultivation.